ABSTRACT
OBJECTIVE: Celiac disease is a permanent enteropathy caused by the ingestion of gluten, which leads to an immunemediated inflammation of the small intestine mucosa. The prevalence of celiac disease varies among different nations and ethnic backgrounds, and its diversity is determined by genetic and environmental factors. São Paulo city is one of the largest cities in the world, with a vast population and an important history of internal migratory flow from other Brazilian regions, as well as immigration from other, primarily European, countries, resulting in significant miscegenation. The aim of the present study was to estimate the prevalence of adults with undiagnosed celiac disease among blood donors of São Paulo by collecting information on the ancestry of the population studied. METHODS: The prevalence of celiac disease was assessed by screening for positive IgA transglutaminase and IgA endomysium antibodies in 4,000 donors (volunteers) in the Fundação Pró-Sangue Blood Center of São Paulo, São Paulo, Brazil. The antibody-positive subjects were asked to undergo a small bowel biopsy. RESULTS: Of the 4,000 subjects, twenty-four had positive tests, although both antibody tests were not always concordant. For example, ten subjects were positive for IgA tissue transglutaminase only. In twenty-one positive patients, duodenal biopsies were performed, and the diagnosis of celiac disease was confirmed in fourteen patients (Marsh criteria modified by Oberhuber). In this group, 67% claimed to have European ancestry, mainly from Italy, Portugal and Spain. CONCLUSION: The prevalence of celiac disease is at least 1:286 among supposedly healthy blood bank volunteers in São Paulo, Brazil.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Blood Donors/statistics & numerical data , Celiac Disease/epidemiology , Blood Banks , Brazil/epidemiology , Celiac Disease/ethnology , Cities/epidemiology , Racial Groups/statistics & numerical data , Epidemiologic Methods , Immunoglobulin A/blood , Transglutaminases/bloodSubject(s)
Humans , Autopsy , Cause of Death , Diagnostic Errors , Quality Control , Reference StandardsABSTRACT
Objetivo: a doença afeta mais de 10 milhões de pessoas na América Latina. Leva a cardiomiopatia dilatada inflamatória em 30% dos pacientes como conseqüência tardia da infecção pelo protozoário Trypanosoma cruzi, com pior prognóstico que as outras cardiomiopatias dilatadas. estudos prévios mostram aumento dos níveis circulantes do fator de necrose tumoral-alfa (TNF-x) em pacientes com cardiomiopatia chagásica crônica. Assim, o objetivo do presente trabalho foi avaliar efeito do bloqueio do TNF-x com Etanercept na função ventricular esquerda em hamsters sírios cronicamente infectados pelo T. cruzi...
Subject(s)
Humans , Animals , Rats , Chagas Cardiomyopathy/veterinary , Animal Experimentation , EchocardiographySubject(s)
Humans , Male , History, 20th Century , History, 21st Century , Pathology/history , PortraitABSTRACT
Cerebral phaeohyphomycosis ("chromoblastomycosis") is a rare intracranial lesion. We report the first human culture-proven case of brain abscesses due to Fonsecaea pedrosoi in Brazil. The patient, a 28 year-old immunocompetent white male, had ocular manifestations and a hypertensive intracranial syndrome. Magnetic resonance imaging (MRI) of the brain revealed a main tumoral mass involving the right temporo-occipital area and another smaller apparently healed lesion at the left occipital lobe. A cerebral biopsy was performed and the pathological report was cerebral chromoblastomycosis. The main lesion was enucleated surgically and culture of the necrotic and suppurative mass grew a fungus identified as Fonsecaea pedrosoi. The patient had received a knife wound sixteen years prior to his hospitalization and, more recently, manifested a pulmonary granulomatous lesion in the right lung with a single non-pigmented form of a fungus present. It was speculated that the fungus might have gained entrance to the host through the skin lesion, although a primary respiratory lesion was not excluded. The patient was discharged from the hospital still with ocular manifestations and on antimycotic therapy and was followed for eight months without disease recurrence. Few months after he had complications of the previous neuro-surgery and died. A complete autopsy was performed and no residual fungal disease was found
Subject(s)
Humans , Male , Adult , Brain Abscess , Central Nervous System Fungal Infections , Chromoblastomycosis , Mitosporic Fungi , Brain Abscess , Central Nervous System Fungal Infections , Chromoblastomycosis , Fatal Outcome , Magnetic Resonance ImagingABSTRACT
A imunomicroscopia eletrônica, feita com anticorpo monocional preparado a partir do Schistosoma mansoni adulto e marcado pelo ouro coloidal, permite a localizaçäo precisa, na biopsia renal, do antígeno esquistossomótico depositado nas estruturas glomerulares. Após terem publicado uma série de pacientes com glomerulopatia esquitossomótico estudados por esse método, os autores atualizam o seu diagnóstico diferencial. A necessidade de excluir as glomerulopatias dos vírus das hepatites B e C, que podem produzir quadro patológicosemelhante ao da glomerulopatia esquitossomótica, é ressaltada. As diferenças com as lesöes renais descritas no Egito säo comentadas (au)
Subject(s)
Humans , Male , Female , Adult , Glomerulonephritis/diagnosis , Glomerulonephritis/etiology , Kidney Diseases , Schistosomiasis mansoniABSTRACT
Pacientes com a doença de Chagas na fase crônica têm sido tratados por meio de transplante de coraçäo, em determinadas circunstâncias, e em virtude de imunodepressäo pode ocorrer reativaçäo da infecçäo devida ao Trypanosoma cruzi. Foi verificado que o alopurinol mostrou-se apto a arrefecer esse acontecimento. Por isso, utilizando modelo experimental, procuramos verificar se tal fármaco é capaz de evitar a reativaçäo parasitária quando usado preventivamente. Verificamos, contudo, que propriedade dessa natureza näo ficou comprovada.
Subject(s)
Animals , Female , Mice , Allopurinol , Chagas Disease , Immunosuppressive Agents , Trypanosoma cruzi , Antiparasitic Agents , Azathioprine , Betamethasone , Cyclosporine , Polymerase Chain Reaction , RecurrenceABSTRACT
A colonizacao do colo e reto por espiroquetas intestinais foi detectada pela primeira vez no Brasil em 4 de 282 (1,41 por cento) pacientes submetidos a sigmoidoscopia e/ou colonoscopia com o diagnostico histopatologico de colite cronica inespecifica. A frequencia e provavelmente menor que a real, uma vez que especimens cirurgicos nao foram considerados no presente estudo. O diagnostico histopatologico foi feito atraves de coloracoes de rotina, tais como a hematoxilina-eosina, que mostraram franja basofilica de 3u de espessura sobre a borda em escova do epitelio e por impregnacoes pela prata como o metodo de Whartin-Starry. Metodos imunohistoquimicos, usando dois soros primarios policlonais, um contra o Treponema pallidum e outro contra Leptospira interrogans, serovar copenhageni serogrupo...
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Colitis/etiology , Spirochaetales Infections/diagnosis , Intestines/parasitology , Immunohistochemistry , Spirochaetales/growth & developmentABSTRACT
Dois antissoros produzidos em carneiros, um dos quais contra a fracao polissacaridica (Po) e outro contra componentes proteicos (Pt) de vermes adultos de Schistosoma mansoni, foram avaliados quanto ao seu desempenho na deteccao de antigenos parasitarios circulantes em pacientes com diferentes formas ciclicas da esquistossomose mansonica, atraves de um ensaio imunoenzimatico, (ELISA). O primeiro antissoro revelava antigenos parasitarios no granuloma hepatico e o segundo, nos glomerulos renais de pacientes com esquistossomose mansonica, bem como em camundongos experimentalmente infectados pelo S. mansoni....
Subject(s)
Humans , Polysaccharides/immunology , Schistosoma mansoni/drug effects , Schistosomiasis mansoni/immunology , Enzyme-Linked Immunosorbent Assay , Oxamniquine/therapeutic use , Polysaccharides/isolation & purification , Praziquantel/therapeutic use , Schistosoma mansoni/metabolism , Schistosomiasis mansoni/drug therapyABSTRACT
Os autores apresentam uma técnica de imunoperoxidase para a identificaçäo de leptospiras em cortes de rim fixados em formol e emblocados em parafina. A variante metodológica inclui a utilizaçäo do complexo peroxidase-antiperoxidase, conferindo alta sensibilidade à reaçäo. Säo, ainda, discutidas algumas possíveis aplicaçöes do método ao estudo do acometimento renal na leptospirose
Subject(s)
Guinea Pigs , Animals , Immunoenzyme Techniques , Leptospira/isolation & purification , Antigens, Bacterial/analysis , Immune Sera , Kidney/microbiology , Leptospira/immunology , Leptospira/microbiologyABSTRACT
O relatório trata da necessidade de reformulaçäo da Lei 10.095 de 03/05/1968 em vigor, do Serviço de Verificaçäo de Obitos e apresenta nova proposta de Lei. Considera que a atual legislaçäo näo contempla adequadamente as atuais demandas no setor de assistência médica, pesquisa, ensino e qualidade de informaçäo do ponto de vista estatístico e propöe alteraçöes essenciais para o aprimoramento da questäo